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. 2015 Dec 30;7(5):5715–5727. doi: 10.18632/oncotarget.6796

Figure 2. Wogonin Inhibited c-Myc Expression and Promoted HIF-1α Degradation in MM cells.

Figure 2

A. RPMI 8226 cells (top) or U266 cells (bottom) were treated with various concentrations of wogonin (0, 20, 40 and 80μM) for 24 h under normoxia and hypoxia. Cell lysates were prepared and subjected to Western blot analysis using the indicated antibodies. B. RPMI 8226 and U266 cells were treated with wogonin (80μM) for 24h under normoxia and hypoxia, and subjected to HIF-1α immunofluorescent staining. Nuclei are counter stained with DAPI (blue). Magnification, 400×. C. RPMI 8226 cells were treated with cycloheximide (CHX) for 0, 10, 30, 60 and 120min under normoxia and hypoxia in the presence or absence of wogonin (80μM). Cell lysates were prepared and subjected to immunoblotting analysis using the indicated antibodies. D. Quantification of HIF-1α protein levels shown in (C). E. RPMI 8226 cells were treated with the proteasome inhibitor, MG132 for 6h under normoxia and hypoxia with or without wogonin. Cell lysates were prepared and subjected to immunoblotting analysis using an anti-HIF-1α antibody. F. Quantification of HIF-1α protein levels shown in (E). Data are shown as means ± SEM (n = 3). *p < 0.05, one-way ANOVA.