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. 2015 Dec 31;7(5):5769–5787. doi: 10.18632/oncotarget.6806

Figure 3. Arsenite-induced neoplastic transformation has effects on the levels of lncRNAs and HIF-2α in L-02 cells.

Figure 3

Abbreviations: L-02, normal L-02 cells, C-L-02, passage control L-02 cells; T-L-02, arsenite-transformed L-02 cells; SMMC-7721, SMMC-7721 carcinoma cells. Densities of bands were quantified by Eagle Eye II software. GAPDH levels, measured in parallel, served as controls. L-02 cells were exposed to 0.0 or 2.0 μM of arsenite for 30 passages. (A) Colonies of normal L-02 cells, passage control L-02 cells, arsenite-transformed L-02 cells, and SMMC-7721 cells and their numbers (B) (means ± SD, n = 3) in soft agar (bars = 250 μm). *P < 0.05 different from medium control cells. (C) At 4 weeks after inoculation of normal L-02 cells, passage control L-02 cells, arsenite-transformed L-02 cells, and SMMC-7721 cells, tumors that formed from the transformed cells and SMMC-7721 cells were examined, and their volumes (D) were measured (means ± SD, n = 6). *P < 0.05 different from medium control cells. L-02 cells were exposed to 0.0 or 2.0 μM of arsenite for 0, 10, 20, or 30 passages. (E) RT-PCR analyses of the mRNA levels of GAS5, lincRNA-p21, H19, HOTAIR, and MALAT1. (F) Western blots for HIF-2α were made for L-02 cells exposed to 0.0 or 2.0 μM arsenite for 0, 3, 6, 12, or 24 h. (G) RT-PCR was performed for GAS5, lincRNA-p21, H19, HOTAIR, and MALAT1, and (H) Western blots for HIF-2α were made.