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. 2016 Jan 7;7(5):5924–5942. doi: 10.18632/oncotarget.6827

Figure 6. C12-induced tumor cell apoptosis is independent of BH-3 only proteins Noxa and Puma.

Figure 6

(A) Noxa expression was stably reduced in HCT116 cells by shRNA. (B) Following 24 hours treatment of C12, similar levels of cell death were detected in HCT116 cells expressing Noxa shRNA and their control counterparts. (C) Less apoptosis was detected in HCT116 cells with reduced Noxa expression upon treatment of cisplatin (50 μM). (D) Caspase-3/7activities were measured after 24 hour-incubation of C12 or cisplatin in the indicated HCT116 cells. (E) Puma expression in the indicated HCT116 cells was examined by western blot. (F) Cell death was measured after 24 hour-incubation of C12 or tunicamycin in Puma-deficient HCT116 cells or wild-type cells. (G) Caspase-3/7 activities were examined 24 hours after C12 or etoposide exposure. All data are presented as mean ± standard deviation of three different experiments. Asterisk indicates P < 0.05 (*) or P < 0.01 (**) by student's unpaired t test. ns, no significant.