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. 2016 Jan 7;7(5):5924–5942. doi: 10.18632/oncotarget.6827

Figure 9. PON3 sensitizes tumor cells with reduced PON2 expression to C12.

Figure 9

(A) Human or murine PON3 cDNA was stably overexpressed in A549 cells with reduced PON2 expression by retroviral infection. Expression levels of PON3 were determined by western blot. (B–C) After treating with different doses of C12 for 24 hours, more cell death (B) and caspase-3/7 activation (C) were detected in PON2-knockdown A549 cells with increased mouse and human PON3 expression comparing to vector control and parental cells. (D) Stable overexpression of human or murine PON3 in PON2-knockdown NCI-H1299 cells was examined by western blot. (E–F) C12 induced more cell death (E) and caspase-3/7 activation (F) in PON2-knockdown NCI-H1299 cells expressing mouse and human PON3 after 24 hours treatment. All data shown are mean ± standard deviation of three independent experiments performed in triplicate. Asterisks indicate P values of < 0.05 (*) or < 0.01 (**) by student's unpaired t test.