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. 2015 Dec 22;7(5):5985–5999. doi: 10.18632/oncotarget.6719

Figure 5. Effect of 5-fluorouracil (5-FU) on expression or apoptosis associated marker in MDA-MB-231 cells.

Figure 5

MDA-MB-231 cells were treated with 5-fluorocytosine (5-FC) at 5.0 μg/ml or 5-FU at 0.5, 1.0, and 5.0 μg/ml for 24 hours, after which BAX and Bcl-2 expression were determined by reverse transcriptase polymerase chain reaction (RT-PCR) analysis. GAPDH was used as a loading control and each sample was normalized to the GAPDH mRNA content. (A) BAX and Bcl-2 RNA expression levels. (B) Graph of BAX gene levels. (C) Graph of Bcl-2/BAX ratio. Data shown are the mean ± SD of three different experiments performed in triplicate. *p < 0.05 vs. negative control (no treatment with 5-FU).