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. 2015 Dec 23;7(5):6175–6187. doi: 10.18632/oncotarget.6742

Figure 4. Down-regulation of FZD7 increased apoptosis induced by IM.

Figure 4

(A) After transduced with specific FZD7 shRNA lentiviral particles for 3 days, K562 cells were treated by imatinib with serial concentrations for additional 48 hrs. MTT assay was conducted to evaluate drug sensitivity. Results were average values of three independent experiments. *P < 0.05. (B and C) Transduced K562 cells were treated by IM (0.2 μM) for 48 hrs and subjected to Annexin V-APC/PI staining for flow cytometry. The percentage of cells was shown in each quadrant. Colums represented means ± S.E. **P < 0.01. (D) Transduced K562 were treated with IM for another 48 hrs. Caspase-3 and PARP-1 were estimated by Western blot. (E and F) Primary CMLcells were transduced with FZD7 shRNA lentiviral particles for 3 days, and then cultured with 5 μM IM for 48 hrs. Cells were subjected to annexin V-APC/PI staining for flow cytometry. The percentage of cells was shown in each quadrant. Colums represented means ± SE (n = 3). *P < 0.05.