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A549 cells with or without LITAF shRNA were treated with metformin (10 mM) for 24 hours and total RNA was isolated for qRT-PCR analysis of miRNAs, miR-194, miR-128, miR-15a, and miR-192. Each sample was amplified in triplicate and normalized to U6 expression. Results were evaluated by the comparative threshold cycle value method for relative quantification of gene expression. Graphs represent mean ± SD (n = 3), student t test,*p < 0.05, **p < 0.01.
