Table 4. Primers used in Q-PCR.
Primer sequences (5′-3′) | |
---|---|
hsa-miR-15a | RT-primer-5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCACAAAC-3′ |
Forward: GCGGCTAGCAGCACATAATGG Reverse: GTCGTATCCAGTGCAGGGTCC | |
hsa-miR-128 | RT-primer: 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAGAG-3′ |
PCR primer: Forward: TCCGATCACAGTGAACCGGT Reverse: GTGCAGGGTCCGAGGT | |
hsa-miR-192 | RT-primer: 5′-GCTGTCAACGATACGCTACGTAACGGCATGACAGTGTTTTTTTTTTTTTTTTTTTTTTTTA-3′ |
PCR primer: Forward: CTGACCTATGAATTGACAGCCA Reverse: GCTGTCAACGATACGCTACGT | |
hsa-miR-194 | RT-primer: 5′-GCTGTCAACGATACGCTACGTAACGGCATGACAGTGTTTTTTTTTTTTTTTTTTTTTTTTG-3′ |
hsa-miR-194 | RT-primer: 5′-GCTGTCAACGATACGCTACGTAACGGCATGACAGTGTTTTTTTTTTTTTTTTTTTTTTTTG-3′ |
U6 | Forward: CGCTTCGGCAGCACATATAC Reverse: TTCACGAATTTGCGTGTCAT |
Bmi-1 | Forward: GCCTTCTCTGCTATGTCTGAA Reverse: CTGATGAACACACACCAACTT |
GAPDH | Forward: CAGGGCTGCTTTTAACTCTGGT Reverse: GATTTTGGAGGGATCTCGCT |