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AML-12 cells were poured in triplicate into 6-well plate with 0.35% agar containing 50 mM ethanol or PBS as control and grown in the medium with or without 50 mM ethanol and plus EGF (20ng/ml) for 4 weeks or longer. The cells were analyzed for colony formation in soft agar. Colonies were counted at 3-4 weeks after plating. Values are the means ± SE (n≥C3). *: p < 0.05 as indicated.
