Table 2.

Primers and programs for quantitative PCR.

	Programs	Primer	Sequences 5′–3′	Reference
Total yeast	95°C 10 s; 60°C 10 s; 72°C 15 s. Cycle: 50	NL1	GCATATCAATAAGCGGAGGAAAAC	Mills et al., 2002
		NL4	GGTCCGTGTTTCAAGACGG
Saccharomyces	95°C 10 s; 60°C 10 s; 72°C 10 s. Cycle: 35	Sac F	GAAAACTCCACAGTGTGTTG
		Sac R	GCTTAAGTGCGCGGTCTTG
Aureobasidium	95°C 10 s; 60°C 10 s; 72°C 10 s. Cycle: 40	Aur F	CGCATCGATGAAGAACGCAG	This study
		Aur R	CAACTAAGGACGGCACCCAA
Rhodotorula	95°C 10 s; 60°C 10 s; 72°C 10 s. Cycle: 50	Rho F	ACCTTGCGCTCCTTGGTATT	This study
		Rho R	TCCTTTAACCCAACTCGGCT
Meyerozyma	95°C 10 s; 60°C 10 s; 72°C 10 s. Cycle: 40	Mey F	AGATAGGTTGGGCCAGAGGT	This study
		Mey R	GCATTTCGCTGCGTTCTTCA
Torulaspora	95°C 10 s; 60°C 10 s; 72°C 10 s. Cycle: 40	Tor F	CAAAGTCATCCAAGCCAGC	This study
		Tor R	TTCTCAAACAATCATGTTTGGTAG
Metschnikowia	95°C 10 s; 60°C 10 s; 72°C 10 s. Cycle: 40	Met F	CAACGCCCTCATCCCAGA	This study
		MetR	AGTGTCTGCTTGCAAGCC
Debaryomyces	95°C 10 s; 57°C 10 s; 72°C 10 s. Cycle: 50	Deb F	TGAAGAACGCAGCGAAATGC	This study
		Deb R	GCCGAGCCTAGAATACCGAG
Hanseniaspora	95°C 10 s; 57°C 10 s; 72°C 10 s. Cycle: 50	HanF	CCCTTTGCCTAAGGTACG	This study
		HanR	CGCTGTTCTCGCTGTGATG