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. 2016 May 17;7:124. doi: 10.3389/fphar.2016.00124

FIGURE 1.

FIGURE 1

Epoxydocosapentaenoic acids (EDPs) preserve HL-1 cardiac cell viability from hypoxia–reoxygenation (H/R) injury. HL-1 cardiac cells were subjected to either 30 h normoxia or 24 h hypoxia and 6 h reoxygenation in the presence of 19,20-EDP (1 μM), docosahexaenoic acid (DHA; 100 μM) and/or (methylsulfonyl)-2-(2-propynyloxy)-benzenehexanamide (MSPPOH; 50 μM). Treatment of HL-1 cells exposed with DHA or EDPs during H/R injury resulted in (A) preserved cell viability, (B) enhanced mitochondrial activity, and (C) better contractility. Furthermore, both (D) proteasomal, and (E) caspase 3/7 activities were attenuated. Values are represented as mean ± SEM; N = 3 independent experiments; ∗p < 0.05 treatment vs. normoxic control, #p < 0.05 treatment group vs. H/R control or DHA/N-MSPPOH.