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. 2016 May 17;7:124. doi: 10.3389/fphar.2016.00124

FIGURE 5.

FIGURE 5

EDPs induce SIRT1 activity in HL-1 cardiac cells. HL-1 cardiac cells were subjected to either 30 h normoxia or 24 h hypoxia and 6h reoxygenation in the presence of 19,20-EDP (1 μM), DHA (100 μM), and/or MSPPOH (50 μM). EDPs increased both (A) intracellular SIRT1 enzymatic activity and (B) NAD+/NADH ratios in HL-1 cells. (C) EDPs and DHA prevented the increase in lactate/pyruvate ratio following H/R injury. Values are represented as mean ± SEM; N = 3 independent experiments; ∗p < 0.05 treatment vs. normoxic control, #p < 0.05 treatment group vs. H/R control or DHA/MSPPOH.