Table 2.

Sensitivity of the different methods determined as the lowest PC concentration exhibiting a response above the threshold.

Technology used	ELISA				MSD				Gyrolab				AlphaLISA
Acid dissociation step	No		Yes		No		Yes		No		Yes		No		Yes
Target depletion step	No	Yes	No	Yes	No	Yes	No	Yes	No	Yes	No	Yes	No	Yes	No	Yes
Final dilution applied before platform analysis	1 in 6		1 in 45		1 in 6		1 in 45		1 in 6		1 in 9		1 in 15		1 in 45
Sensitivity ng/mL of reference ADA	5		100		2		20		2		5		20		20