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. 2016 Mar 1;35(10):1098–1114. doi: 10.15252/embj.201592701

Figure EV1. Southern blotting analysis of homologous recombination.

Figure EV1

Southern blot analysis of genomic DNA from homozygous wild‐type (RBE wt) and mutated alleles of RIBEYE (RBE KI+neo, RBE KI, and RBE KO; see Fig 1A). Correct recombination events were confirmed using outside probes hybridizing to the 5′ (SacI‐digested genomic DNA) and the 3′ homology region (BamHI digested) and to the GFP‐coding portion of GCamP3 (Venus probe; BamHI digested). Positions of size markers are indicated on the right.