Skip to main content
. 2016 May 17;6:26178. doi: 10.1038/srep26178

Figure 5. Prolonged DNA damage induced foci formation in ΔRNF4 cells is suppressed by UBE2W inactivation.

Figure 5

After treatments with or without MMC (50ng/ml) for 1H, cells were allowed to recover for 4H or 16H. (a) For each indicated cell line, the formation of MMC induced foci for γ-H2ax (green) and Rad51 (red) was analysed by immunostaining. Chromatin was visualized by DAPI (Blue). Representative images are shown for each staining and merged images. White bar: 10 um. (b,c) percentage of cell containing no focus; 1–5 foci; >5 foci are represented for each conditions respectively in blue, red and yellow. (a) γ-H2ax. (b) Rad51 quantification. More than 100 cells have been counted for each condition.