Figure 3.

Effect of ChCl/EG (1:2) content (a) and substrate concentration (b) on the product yield (□), product e.e. (○) and initial reaction rate (△) of the biosynthesis of (R)-2-octanol with the biocatalyst in monophasic system. Reaction conditions: (a) TEA-HCl buffer (50 mM, pH 5.0, 10.0 mL) containing different content of ChCl/EG (v/v), 2-octanone (40 mM), isopropanol (500 mM), Acetobacter pasteurianus GIM1.158 cell (25 mg mL⁻¹), 35 °C, 180 rpm; (b) TEA-HCl buffer (50 mM, pH 5.0, 10.0 mL) containing of ChCl/EG (40%, v/v), different concentration of 2-octanone, isopropanol (500 mM), Acetobacter pasteurianus GIM1.158 cell (25 mg mL⁻¹), 35 °C, 180 rpm.