Table 1. Primers Designed for Real-time qPCR.

Gene of interest	Oligonucleotide sequence 5′→3′
ADAM17	F—GTTCGCATTAAGCCGTTCC	R—TGGGGTCTTCCTGGATGGTG
CYP11B1	F—TGCCTGAGGACGCTGAGAAG	R—AGTCGGTTGAAGCGCCAGTC
IL-1β	F—GTGTCACTCATTGTGGCTGTGG	R—GGGAACATCACACACTAGCAGGTC
IL-6	F—AGAAAAGAGTTGTGCAATGGCA	R—GGCAAATTTCCTGGTTATATCC
TNFα	F—CAAGGAGGAGAAGTTCCCA	R—TTGGTGGTTTGCTACGACG
TLR2	F—CCCTCAGTCTTGGAGTGTCACCAG	R—GGAGCTTTCTTGGGCTTCCTC
TLR4	F—CCAGAATGAGGACTGGGTGAGAAA	R—CCACCACAATAACTTTCCGGCTCTTG
INSR	F—GCTTCTGCCAAGACCTTCAC	R—TAGGACAGGGTCCCAGACAC
INS2	F—CAGCACCTTTGTGGTTCTCA	R—CAGTGCCAAGGTCTGAAGGT
NLRP3	F—CAGGGCTAAAGCAGCAGATGG	R—TCCGCAGCCAATGAACAGAG
HMBS	F—GGAGGTCCGAGCCAAGGACCAG	R—CTGGCACGCTACAGCCTCCTTCC

Abbreviations: IL-1β, interleukin-1β; IL-6, interleukin 6; TLR2, Toll-like receptor 2; TLR4, Toll-like receptor 4; TNFα, tumor necrosis factor α.