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. 2016 May 17;6:26092. doi: 10.1038/srep26092

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Copyright © 2016, Macmillan Publishers Limited

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RNAi-mediated knockdown of Kr-h1 was carried out in N4. Control insects injected with malE dsRNA molted into fifth instar nymphs. Ovary development was reduced in control N5 (a–c). Ovaries (d–f) form Kr-h1 dsRNA injected insects showed the precocious development of ovaries (d–f). These ovaries showed well-developed ovarioles (e) where the germarium (green arrowhead) is detected. Lateral oviduct and common oviduct are also developed (f). These structures including the germarium (green arrowhead) and oocytes (red arrowhead) are well developed in control adult (g–i). OVA, ovarioles; LOV, lateral oviduct; COV, common oviduct and SC, seminal conceptacle. Bar represents 100 μm.