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. 2016 May 17;6:26092. doi: 10.1038/srep26092

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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RNAi-mediated knock down of Kr-h1 was carried out in N4. Control insects injected with malE dsRNA were dissected to observe the development of testis (a–c). Kr-h1 knockdown insects showed the development of male reproductive organ (d–f). Vas-deference (VD), seminal vesicle (SV), male accessory gland reservoir (Red arrow head) and male accessory glands (MAG) were observed in kr-h1 knockdown males. Male reproductive organs dissected from control adults are shown in (g–i) for comparison. Bar represents 100 μm.