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. 2016 May 17;2:16008. doi: 10.1038/celldisc.2016.8

Figure 4.

Figure 4

MLL1 interaction with WDR5 is required for MLL1 recruitment at a subset of genes. (a) Summary for changes in MLL1 or WDR5 binding after either Mll1 deletion or MM-401 treatment. (b) Venn diagram of overlap of changes in WDR5 binding upon Mll1 deletion or MM-401 treatment. (c) Pie chart of changes in MLL1 or WDR5 binding after MM-401 treatment. Relative ratio of each category versus total peaks was indicated as %. (d) ChIP assay for MLL1, WDR5 and H3K4me2 at selected gene loci as indicated on bottom. Signals for each experiment were normalized to 5% input. Means and s.d. (as error bars) from at least three independent experiments were presented. Four groups of genes were selected based on MM-401 induced changes in MLL1 and/or WDR5. (I) No change in MLL1 and WDR5 binding; (II) MLL1 binding is disrupted; (III) WDR5 binding was disrupted; and (IV) both WDR5 and MLL1 binding were disrupted. Student t-test were performed for statistical analyses, *P<0.05, **P<0.01. (e) Gene ontology term analyses on MLL1 direct targets that have disrupted MLL1 binding after inhibitor treatment.