Fig. 4.

Validation of ketamine-induced gene expression alterations. The expression of Plin4, Sgk1 and Tsc22d3 in response to ketamine was measured in the mouse striatum. a Ketamine-induced expression of specific transcriptional variants was analyzed using next-generation sequencing (n = 4). Variant classification indicated different biotypes of regulated transcripts (depicted in red) (Additional file 6). b The changes in mRNA abundance levels were measured 2 h after the administration of ketamine, saline or in naive animals. qPCR analyses were performed using samples from an independent biological experiment (n = 8). TaqMan probes allow to distinguish between specific transcriptional variants: qPCR probe “1” spans an exon junction of exons 4 and 5 of Plin4 (ENSMUST00000002908) transcript, qPCR probe “2” spans exons 1 and 2 in Sgk1 (ENSMUST00000120509), qPCR probe “3” spans exons 1 and 2 in Sgk1 (ENSMUST00000020145), qPCR probe “4” span exons 1 and 2 in Tsc22d3 (ENSMUST00000112995), qPCR probe “5” spans exons 1 and 2 in Tsc22d3 (ENSMUST00000055738). Bars indicate S.E.M, *** p < 0.001, one-way ANOVA drug factor