Fig. 4.

Effects of LY294002 (LY) and compound C (CC) on glycogen synthesis and TG content in HepG2 cells. HepG2 cells were incubated in high-glucose medium (33 mM) for 16 h and then pretreated with RCE for 12 h or kinase inhibitors (20 μM LY294002 or 10 μM compound C) prior to RCE treatment (15 μg/mL) for 1 h. Glycogen synthesis (a) and TG content (b) were measured. Expression levels of p-ACC, SREBP1c, and p-GSK3ß were measured by western blotting (c). A quantitative analysis of the protein levels of p-ACC (d), SREBP1c (e), and p-GSK3ß (f) was conducted. The results represent the mean ± SEM (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 vs. the sample without RCE; #p < 0.05, ## p < 0.01, and ###p < 0.001 vs. the RCE-treated sample