Table 3.

Primers used for qRT-PCR validation

Unigenes	KEEG Annotation	Gene	Forward 5'–3'	Reverse 5'–3'
CL12551Contig1	Apoptosis	Serine/threonine-protein kinase	TATTCCCTTTTCCGATCTTCGCTAT	CAAAGATGAGTTGCTTTTCGTGTTG
CL23964Contig1	Calcium signaling pathway	calcineurin B-like protein	AAATTTGCATATCTGCCTGTGTCAA	ATGTAAAACATCCAAAACCCCAGTC
CL32645Contig1	Flavonoid biosynthesis	ANR	AGTATGGCTTTTGTGCTTTGATTGA	TAGAGGTGTCAAGGTTTCAGTTTCA
CL28991Contig1	Flavonoid biosynthesis	CYP75A(F3'5'H)	TTGTAAGTATGGGAGTTTGGGTAGG	CGACACATCATCAAGCGTAATAGAG
CL26013Contig1	Flavonoid biosynthesis	LDOX	AGTTGGCTAATAATGCTAGTGGTCA	TTTTCTAGTCGGCCTTCTTCTAGTC
CL20835Contig1	negative regulation of programmed cell death	Probable WRKY 40	ATTGTACTTGTCGCAAATGTCTGTT	CAATGTTAACCCACTTCCACTACAC
CL1Contig2502	Plant-pathogen interaction	caffeoyl-CoA O-methyltransferase	TCACAAGAACCAGACACAAACATTC	TTAATGGGCACAAGGGTTATGTTTC
CL41260Contig1	Plant-pathogen interaction	CPK	GATTCCTAAACATTTCCAAGCCACA	CTTTTAATGGTTGGACGGTGAGAAA
CL25650Contig1	Plant-pathogen interaction	PR1	ACCGTTATTTGTACACTGAACCCTA	TATGCTAATTCGAGGATGGGTGATT
CL21816Contig1	Plant-pathogen interaction	RPS2	CCAATGGGACCTCTGAACTATTTTG	TGCTTAAATTGCCTTTCAACGAAGA
CL2609Contig1	Plant-pathogen interaction	CALM	TGAAATCTATCATTCCATCCCCGTT	GAAGGGAGCTTTTGATGTGTTTGAT
CL1Contig5289	ubiquitin mediated proteolysis	Skp1	TGGGTTCCAATGGTATTGTTTGAAG	CGAGTGGATTATCGTTCAGTTGATG
CL1Contig1054	ubiquitin mediated proteolysis	Cullin-1	ATATGTGTGGTAACCCTATTGGTCC	AATTCCCCGTAAACCAGTTCATAGA
CL25362Contig1	Response to auxin	Protein TIFY 9	ATTGACGATTTTCTACAACGGAACC	TCTCCAGAAGTTGATGTTGATCTGT
CL31783Contig1	Response to salicylic acid	12-oxophytodienoate reductase 11	TCATAACAAGGGTGGGATCTTCTTT	CACTGTATCTTTCATGAACTGGTCG
CL25983Contig1	-	Ribonuclease, T2 family	CTGCGATAGCCGCAACTCTT	GGAAGTAGCTGTGCTGGTCAA
GE651107	Reference gene	GAPDH	GGAAGCATCATGAACTCAAAGTGAA	ATCCTTCTCATTGACACCCATAACA