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. 2016 May 17;9:286. doi: 10.1186/s13071-016-1568-4

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© Long et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Differential expression of Ac-cathB-2 gene in the life cycle of Angiostrongylus cantonensis. a Quantitative real-time PCR (qRT-PCR) was used to measure the expression of Ac-cathB-2 gene throughout the life-cycle stages of A. cantonensis, with β-actin as an internal reference. The data are presented as relative quantities and the average amount of Ac-cathB-2 gene expression in male adults was taken as 1. b Equal amounts of ESPs from L3 (Lane 1), female adults (Lane 2), male adults (Lane 3), female L5 (Lane 4) and male L5 (Lane 5) were subjected to western blot assay. The native Ac-cathB-2 in these samples was visualised with the antiserum against Ac-cathB-2