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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Child Dev. 2016 Jan;87(1):86–97. doi: 10.1111/cdev.12484

Table 2.

Pyrosequencing Primers and Assay Sequences

NR3C1 Assay Name
(CpG Positions)		 Primers* Sequence to analyze
(converted)
Chromosome location
1D
(8)		 PCR forward GGATAAGAGGTTTGTTGAAAGTTTATT
PCR reverse Biotin-ACTCCCCCTACTCTAACAT
Forward sequencing 1D AGGAAGGAAGGTTTAGGT		 5:142785061-142785008
TATTYGG/AYGATYGYGAATTTTTGTTAAGATGKTGGTYGYGGGGAYGGGTTGGYGATATTGTATTTTATTAAGATGG
1F
3 pyrosequencing assays
(19)		 PCR forward TTTTTTTTTTGAAGTTTTTTT
PCR reverse Biotin-CCCCCAACTCCCCAAAAA
Forward sequencing F0 GAGGAGTTTAGGTTTTTGTG
Forward sequencing F1 GAGTGGTTTGGAGT
Forward sequencing F2 AGAAAAGAATTGGAGAAATT 		sequence F0 (CpG 1-6) 5:142783720-142783676 GTTYGTTYGTTGTTATTYGTAGGGGTATTGGYGG/AYGTTTGTYGTTAAGGGGTAGAG
sequence F1 (CpG 7–11) 5:142783640-142783588 YGYGGAGTTGGGYGGGGGYGGGAAGGAGGTAGYGAGAAAAGAAATTGGAGAAA
sequence F2 (CpG 12–19) 5:142783585-142783501 YGGTGGTTTTTTTAAYGTYGTTTTAGAGAGATTAGGTYGGTTTTYGTYGTTGTYGTYGTTGTTTTTTTTTTTGGGGAGTTGGGGG
1H
(9)		 PCR forward AGGGGTTTTTTTTTTATTTATGGAGA
PCR reverse Biotin-CCCACTCCCCCAAACTAATAAAAATTTAT

Forward sequencing 1H GAGAGGTTTTTTTGTTAGAGGTA		 5:142782749-142782607
AGAAGYGAGGYGGRAGGGGGTYGGGGYGYGTTYGTTTTTTYGAGGTGTYGTTGGGATYGGAGATAA
*

Primer sequences are given 5′ to 3′ direction