Fig. 3.

Accumulation of Aβ in fly heads throughout aging. The levels of soluble Aβ42 (panel A; extracted with 1% w/v SDS in PBS) and total Aβ42 (panel B; extracted with 5 M GnHCl) were measured as the flies aged. The levels in the absence of Aβ42 induction (baseline reading) were compared with flies induced continually (black), for days 1–15 (green) and from day 15 onward (red). Error bars show the standard deviation of 3 biological replicates. GnHCl interference with the assay may result in under-estimation of total A levels in panel B, however, comparisons within panel B are valid. Abbreviation: Aβ, amyloid beta; SDS, sodium dodecyl sulfate.