Fig. 4.

Aβ coexpression does not exacerbate the age-related heat sensitivity of Drosophila shibireTS2. Titration of temperature with climbing performance of shibireTS2 flies at different ages demonstrated that although Drosophila homozygous for shibireTS2 became paralyzed at increasingly lower temperatures as they aged (panels A through to C), the coexpression of Aβ did not exacerbate this paralytic phenotype. The locomotor assessment was carried out with 3 biological replicates per condition. The video assessment of walking velocity was undertaken as described previously (Jahn et al., 2011). A 2-way ANOVA analysis of the climbing performance did not show any differences between the genotypes. The flies were crossed and maintained at 18 °C before each locomotor assessment. The 1x shibireTS2 negative control data (rectangles) for all panels represents the behavior of the oldest day 35 flies. Abbreviations: ANOVA, analysis of variance; Aβ, amyloid beta.