Figure 4.
Modulation of TLR4-induced cytokine release by iC3b-guinea pig red blood cells (gRBCs). Murine monocytes (n = 3) (top), day 2 monocyte-derived macrophages (n = 3) (middle), and day 7 bone marrow–derived macrophages (n = 6) (bottom) were fed with iC3b-gRBCs (at a 10:1 ratio, gRBCs/cells) 1 hour prior to lipopolysaccharide (LPS) stimulation (10 ng/ml) for 24 hours. The amounts of interleukin-6 (IL-6) and IL-10 in the samples with and without CR3 pre-engagement are shown with the P values indicated. Data are expressed as mean ± SEM, paired t-test. The data are representative of 3 independent experiments.
