Figure 1.

Establishment of CPE-silencing osteosarcoma cell line.

Notes: (A) Western blot analysis of CPE protein levels in MG-63, U2-OS and Saos-2 cells. (B) Real-time PCR and (C) Western blot analysis of the expression levels of CPE mRNA and protein in parental, control shRNA, and CPE shRNA-transfected MG-63 cells. Relative CPE expression level was quantified by grayscale analysis with normalization to β-actin. Data are presented as the mean ± standard deviation of triplicate experiments. Compared with the control group, ^***P<0.001.

Abbreviations: CPE, carboxypeptidase E; PCR, polymerase chain reaction; mRNA, messenger ribonucleic acid; shRNA, short hairpin ribonucleic acid.