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. 2016 May 10;9:2795–2803. doi: 10.2147/OTT.S98991

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© 2016 Fan et al. This work is published and licensed by Dove Medical Press Limited

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Silencing of CPE inhibits osteosarcoma cell proliferation and induces cell cycle arrest.

Notes: (A) Cell proliferation was assessed over 12–96 hours by MTT assay. (B, C) Cell proliferation was evaluated by colony formation assay, and the colony formation efficiency was calculated. (D, E) Cell cycle distribution of the parental MG-63 cells, control shRNA cells, and CPE shRNA cells was assessed using flow cytometry and the proportions of cells in G₀/G₁, S, and G₂/M phase were calculated. (F) Expression levels of cyclin D₁ in three group cells were determined by Western blot analysis, with β-actin as the internal control for grayscale comparison. Data are presented as the mean ± standard deviation of triplicate experiments. Compared with the control group, ^*P<0.05, ^**P<0.01.

Abbreviations: CPE, carboxypeptidase E; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; OD, optical density; shRNA, short hairpin ribonucleic acid.

Silencing of CPE inhibits osteosarcoma cell proliferation and induces cell cycle arrest.

Notes: (A) Cell proliferation was assessed over 12–96 hours by MTT assay. (B, C) Cell proliferation was evaluated by colony formation assay, and the colony formation efficiency was calculated. (D, E) Cell cycle distribution of the parental MG-63 cells, control shRNA cells, and CPE shRNA cells was assessed using flow cytometry and the proportions of cells in G₀/G₁, S, and G₂/M phase were calculated. (F) Expression levels of cyclin D₁ in three group cells were determined by Western blot analysis, with β-actin as the internal control for grayscale comparison. Data are presented as the mean ± standard deviation of triplicate experiments. Compared with the control group, ^*P<0.05, ^**P<0.01.

Abbreviations: CPE, carboxypeptidase E; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; OD, optical density; shRNA, short hairpin ribonucleic acid.