Figure 2.

Detection of hexon protein of adenovirus adsorbed onto the anionic polymer-coated magnetic beads from diluted recombinant adenovirus Type 6.

Notes: Recombinant adenovirus Type 6 (Tonsil99; 20 μL, 0.05 mg/mL) was diluted with PBS (500 μL) and then incubated with anionic magnetic beads coated with anionic polymer, poly(MVE-MA). Quick Navi-Adeno was used for the detection of adenovirus capsid protein, hexon by immunochromatography. The presence of adenovirus hexon protein was interpreted on the basis of the presence or absence of a test line (T). A positive control was also included (C). Samples were divided into the following: BD, sample before incubation with the beads (BF), supernatant after incubation with the beads (SP), and samples containing the same quantity of adenovirus as in the BD (total fraction, TL). In each case, samples were solubilized with lysis buffer and subjected to immunochromatography for detection.

Abbreviations: BD, bead fraction; PBS, phosphate-buffered solution; poly(MVE-MA), poly(methyl vinyl ether-maleic anhydrate).