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. 2016 May 12;11:2053–2067. doi: 10.2147/IJN.S102844

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© 2016 Wang et al. This work is published and licensed by Dove Medical Press Limited

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LSCM images of 3T3-E1 cells seeded on BMC, TMC, and NMC scaffolds after culturing for 12 hours (immunocytochemical staining), 1, 3, 5, and 7 days (AO/EB staining).

Notes: Column “IF” shows the F-actin cytoskeleton (green) and nuclei (blue) of preosteoblasts grown on different collagen scaffolds.

Abbreviations: AO/EB, acridine orange/ethidium bromide; BMC, biomimetic mineralized collagen; NMC, non-mineralized collagen; TMC, traditional mineralized collagen; d, days; LSCM, Laser scanning confocal microscopy.