Table 1.

The primers used in qRT-PCR

Gene	Primer sequence	Annealing temperature (°C)	Fragment size (bp)
GAPDH	F:5′-GTTGTCTCCTGCGACTTCA3′	60	293
	R:5′-GCCCCTCCTGTTATTATGG3′
SIRT1	F:5′-GGAACCTTTGCCTCATCTACAT3′	60	164
	R:5′-ATTCCTTTTGTGGGCGTGG3′
Caspase-3	F:5′-TATCCTGAAATGGGCATATG3′	60	140
	R:5′-TTCCTGACTTGGTATTTCAG3′
FOXO1	F:5′-AGTGGATGGTGAAGAGCGT3′	60	324
	R:5′-GCCACTTAGAAAACTGAGACC3′
FasL	F:5′-GGTGCTTGCTGGCTCACAGT3′	60	88
	R:5′-TTCACGAACCCGCCTCCTC3′
BAX	F:5′-CATCGAGGTGGAGAGCGACG3′	60	140
	R:5′-GTGATGGGACTGAGTCCCGC3′
Bcl-2	F:5′-AGAAGAAGGGAGAATCACAG3′	60	160
	R:5′-CGGCATGATCTTCTGTCAAG3′

Abbreviations: F, forward; qRT-PCR, quantitative reverse transcription–polymerase chain reaction; R, reverse.