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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Apr 19;1861(7):671–679. doi: 10.1016/j.bbalip.2016.04.014

FIGURE 1. Endothelial NOS at serine 1177 is selectively phosphorylated based upon endothelial or smooth muscle cell receptor activation.

FIGURE 1

Transverse sections of the VCCC were incubated with antibodies to alpha 1 adrenergic receptor (α1AR, green), bradykinin receptor 2 (BKR2, purple) and endothelial nitric oxide synthase (eNOS, red). SMC only shows α1AR expression while EC only shows BKR2 expression. Endothelial NOS is found in the apical EC as well as the MEJ (A). Immunocytochemistry of VCCC with antibodies against phosphorylated eNOS at S1177 (eNOS-P, red) after stimulation with BK or PE for 1 minute (B). Representative western blot of VCCC fractions after treatment with bradykinin (BK, 10µM, n=4) (C) or phenylephrine (PE, 50µM, n=5) (D) at 1 and 5 minute timepoints, with no treatment as control. The ratio of eNOS-P to total eNOS is presented. All error bars represent SEM. In C and D, * indicates p<0.05. Scale bar for A and B is 10 µm.