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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Apr 19;1861(7):671–679. doi: 10.1016/j.bbalip.2016.04.014

FIGURE 2. Gap junction inhibition prevents phenylephrine induced eNOS phosphorylation at the MEJ.

FIGURE 2

The VCCC was incubated with the gap junction inhibitor 18-GA (50µM) for 30 minutes before adding bradykinin (BK, 10µM, n=4) (A) or phenylephrine (PE, 50µM, n=3) (B). Timepoints indicate the minutes after stimulation. Cellular fractions were harvested for western blotting and the ratio of eNOS S 1177 to total eNOS is presented. All error bars represent SEM. In A and B, * indicates p<0.05