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. Author manuscript; available in PMC: 2016 May 17.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2011 Sep;CHAPTER 2:Unit2D.7. doi: 10.1002/9780470151808.sc02d07s18

Figure 4.

Figure 4

Differentiation of medial ganglionic eminence-neural stem cells (MGE-NSCs) after their dissociation from CldU-labeled neurospheres and incubation in the differentiation medium for 4 days (A1-D3) or eight days (E1-F2). Differentiation of fractions of MGE-NSCs into TuJ-1+ neurons (A1, E1), O1+ oligodendrocytes (B1, F2), and GFAP+ astrocytes (C1, F1) could be seen at both time-points. Furthermore, fractions of MGE-NSCs also differentiate into GABA-ergic neurons (arrows in D1-D3 and E1-E2). Scale bar: 50 μm. The bar charts (G1, H1) illustrate percentages of MGE-NSCs that exhibit differentiation into TuJ-1+ neurons, GABA+ neurons, O1+ oligodendrocytes and GFAP+ astrocytes after incubation in the differentiation medium for 4 days (G1) or 8 days (H1). Note that the expression of GABA among TuJ-1+ neuronal population is ~70% after 4 days and ~50% after 8 days of incubation in the differentiation medium. [Reproduced from: Waldau et al., Stem Cells, 28:1153–1164].