Table 2D.7.1.

Combinations of Various Primary and Secondary Antibodies Used in Single and Dual Immunofluorescence Staining^a

Name	Blocking serum	Primary antibody, sources, and dilution	Secondary antibodyb
BrdU staining	NHS	Mouse anti-BrdU (BD Bioscience), 1:200	Biotinylated anti–mouse IgG (Vector Labs) followed by ABC reagent and DAB
DCX staining	NHS	Goat anti-DCX (Santa Cruz Biotechnology), 1:200	Biotinylated anti–goat IgG (Vector Labs) followed by ABC reagent and VG
BrdU-NeuN dual immunofluorescence	NGS and NDS	Rat anti-BrdU (Serotec), 1:200 Mouse anti-NeuN (Millipore), 1:1000	Goat anti–rat IgG with Alexa Fluor 594 Donkey anti–mouse IgG with Alexa Fluor 488
BrdU-GABA dual immunofluorescence	NGS	Rat anti-BrdU (Serotec), 1:200 Rabbit anti-GABA (Sigma), 1:5000	Goat anti–rat IgG with Alexa Fluor 594 Goat anti–rabbit IgG with Alexa Fluor 488
BrdU-GFAP dual immunofluorescence	NGS	Rat anti-BrdU (Serotec), 1:200 Rabbit anti-GFAP (Sigma), 1:1000	Goat anti–rat IgG with Alexa Fluor 594 Goat anti–rabbit IgG with Alexa Fluor 488
BrdU-s100β dual immunofluorescence	NGS	Rat anti-BrdU (Serotec), 1:200 Rabbit anti-s100β (Millipore), 1:1000	Goat anti–rat IgG with Alexa Fluor 594 Goat anti–rabbit IgG with Alexa Fluor 488
BrdU-O1 dual immunofluorescence	NDS	Rat anti-BrdU (Serotec), 1:200 Mouse anti-O1 (Millipore), 1:1000	Goat anti–rat IgG with Alexa Fluor 594 Donkey anti–mouse IgG with Alexa Fluor 488
BrdU-NG2 dual immunofluorescence	NGS	Rat anti-BrdU (Serotec), 1:200 Rabbit anti-NG2 (Millipore), 1:1000	Goat anti–rat IgG with Alexa Fluor 594 Goat anti–rabbit IgG with Alexa Fluor 488
BrdU-Sox2 dual immunofluorescence	NGS	Rat anti-BrdU (Serotec), 1:200 Rabbit anti-Sox2 (Millipore), 1:1000	Goat anti–rat IgG with Alexa Fluor 594 Goat anti–rabbit IgG with Alexa Fluor 488
Tuj1-GABA dual immunofluorescence	NGS	Mouse anti-Tuj1 (Covance), 1:1000 Rabbit anti-GABA (Millipore), 1:1000	Goat anti–rat IgG with Alexa Fluor 594 Goat anti–rabbit IgG with Alexa Fluor 488
Tuj1-GFAP dual immunofluorescence	NGS	Mouse anti-Tuj1 (Covance), 1:1000 Rabbit anti-GFAP (Millipore), 1:1000	Goat anti–rat IgG with Alexa Fluor 594 Goat anti–rabbit IgG with Alexa Fluor 488
O1-S100β dual immunofluorescence	NGS	Mouse anti-O1 (R&D), 1:500 Rabbit anti-S100β (Millipore), 1:1000	Goat anti–rat IgG with Alexa Fluor 594 Goat anti–rabbit IgG with Alexa Fluor 488
S100β-GDNF dual immunofluorescence	NGS	Mouse anti-S100β (Millipore), 1:1000 Rabbit anti-GDNF (Santa Cruz Biotechnology), 1:1000	Goat anti–rat IgG with Alexa Fluor 594 Goat anti–rabbit IgG with Alexa Fluor 488

^aAbbreviations: NHS, normal horse serum; NGS, normal goat serum; NDS normal donkey serum.

^bSecondary antibodies purchased from Invitrogen were used at 1:200 dilution in all staining described in this unit.