Table 2.
Effect of G2373A gw2-A1 allele on thousand grain weight (TGW) and grain morphometric parameters in tetraploid BC2 and BC4 lines
| Experiment | Genotype (allele) | N | TGW (g) | Area (mm2) | Width (mm) | Length (mm) |
|---|---|---|---|---|---|---|
| BC2F2 (GH) | GW2-A1/GW2-A1 (Kronos) | 15 | 51.4 ± 0.8 | 23.6 ± 0.2 | 3.91 ± 0.02 | 7.79 ± 0.03 |
| gw2-A1/gw2-A1 (G2373A) | 10 | 55.2 ± 1.3 | 24.8 ± 0.2 | 4.06 ± 0.03 | 7.93 ± 0.04 | |
| 7.5 %**,a | 5.2 %** | 3.8 %** | 1.8 %** | |||
| BC2F3 (GH) | GW2-A1/GW2-A1 (Kronos) | 121 | 50.5 ± 0.4 | 22.2 ± 0.1 | 3.95 ± 0.01 | 7.21 ± 0.02 |
| gw2-A1/gw2-A1 (G2373A) | 110 | 53.2 ± 0.4 | 23.2 ± 0.1 | 4.08 ± 0.01 | 7.32 ± 0.02 | |
| 5.4 %*** | 4.4 %*** | 3.2 %*** | 1.6 %*** | |||
| BC4F2 (GH) | GW2-A1/GW2-A1 (Kronos) | 18 | 72.3 ± 1.3 | 27.5 ± 0.3 | 4.28 ± 0.03 | 8.32 ± 0.05 |
| gw2-A1/gw2-A1 (G2373A) | 16 | 75.5 ± 1.2 | 29.2 ± 0.3 | 4.42 ± 0.03 | 8.57 ± 0.05 | |
| 4.4 % | 6.1 % ** | 3.1 % * | 3.0 % ** | |||
| BC2F4 (Field-14) | GW2-A1/GW2-A1 (Kronos) | 11 | 51.3 ± 0.9 | 23.0 ± 0.2 | 3.75 ± 0.02 | 7.76 ± 0.03 |
| gw2-A1/gw2-A1 (G2373A) | 8 | 54.9 ± 0.8 | 24.2 ± 0.3 | 3.88 ± 0.03 | 7.96 ± 0.06 | |
| 7.0 %** | 5.5 %*** | 3.5 %** | 2.6 %** | |||
| BC2F5 (Field-15) | GW2-A1/GW2-A1 (Kronos) | 15 | 58.7 ± 1.0 | 22.9 ± 0.2 | 3.60 ± 0.03 | 8.32 ± 0.03 |
| gw2-A1/gw2-A1 (G2373A) | 18 | 62.5 ± 0.8 | 24.1 ± 0.3 | 3.73 ± 0.02 | 8.47 ± 0.03 | |
| 6.4 %** | 5.5 %** | 3.7 %** | 1.7 %** | |||
| Overall ANOVA BC populations (P value) | <0.001 | <0.001 | <0.001 | <0.001 | ||
Experiments were conducted across glasshouse (GH) and field environments (2014 and 2015). N corresponds to number of plants (GH) or plots (field)
* (P < 0.05), ** (P < 0.01), *** (P < 0.001)
a The percentages correspond to the difference between the gw2-A1 and GW2-A1 phenotypes as a percentage of the GW2-A1 wild type Kronos allele