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Sprouting angiogenesis and anastomosis. (A) Formation of an EC monolayer on the gel interface at the communication pore after EC lining. (B) Invasion of microvascular sprouts from the lined EC monolayer into the gel after 24 hours. (C) More sprouts and deep invasion after 48 hours post-lining. (D) Tight interconnection between EC lining along the outer channel and the capillary network inside tissue chamber by anastomosis with bidirectional migration. Scale bars: 100μm.
