Effects of miR-214 and miR-218 on the cell cycle of breast cancer. After transfection, the cells were washed with PBS twice, and treated with trypsin. The surviving cells were collected, excepting apoptotic cells, in culture medium. The cell cycles were analyzed by flow cytometry after staining with PI. The representative results of cell cycles are shown in (A) negative control, (B) miR-214 mimic and (C) miR-218 mimic transfected cells. (D) In addition, the percentage of cells in the G0/G1, S and G2/M phases was, respectively, evaluated. The experiments were performed in triplicate. *P=0.0298 (the percentage of S phase cells, miR-214 mimic transfected cells vs. negative control cells). &P=0.0009 (the percentage of S phase cells, miR-218 mimic transfected cells vs. negative control cells). #P=0.006 (the percentage of G0/G1 phase cells, miR-218 mimic transfected cells vs. negative control cells).