Table 3.

Fluorescence lifetimes measured from different fluorescent microspheres. Values were calculated from the phase shifts at each harmonic frequency, when measured.

Fundamental frequency (harmonics) [MHz]	5 (15,25,35 45, 55)	6.25 (18.75, 31.25, 43.75)	12.5 (37.5, 62.5, 87.5)	25 (75)
Fluorescence lifetimes [ns] from each frequency
Flow-Check™	7.0 (7.0, 6.9, 7.12, 7.18)	6.99 (6.99, 6.73, 6.94, 5.03)	7.06 (6.71, 5.9, 6.14)	7.02 (7.3)
Fluorescein	4.21 (5.5, 3.71, 2.8, 3.2)	3.73 (4.3, 2.9, 2.31, 2.6)	3.89 (2.33, 3.43, 5.8)	---
Alexa Fluor® 488	4.17 (3.9, 3.84, 3.29, 3.23)	25.1 (8.1, 5.1, 0.89, 0.35)	3.62 (1.99, 4.2, 3.66)	---
Acridine Orange	4.03 (4.08, 3.77, 2.61)	16.1 (4.2, 3.5, 1.3, 0.48)	3.87 (2.59, 2,65, 2,62)	3.99 (2.29)