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. Author manuscript; available in PMC: 2016 May 17.
Published in final edited form as: Gut. 2014 Dec 10;65(1):100–111. doi: 10.1136/gutjnl-2014-308043

Figure 8.

Figure 8

Lipolysis of triolein unlike interleukin (IL)-1β+keratinocyte chemoattractant (KC)/growth-regulated oncogene (GRO) coadministration results in necro-apoptotic injury and renal failure during caerulein (CER) pancreatitis: images of terminal deoxynucleotidyl transferase dUTP nick end labelling (A–E) and immunohistochemistry for kidney injury molecule-1 (A1–E1) in kidneys, with each group mentioned above the image. There was positive staining in the CER+triolein group, sloughing of renal tubular cells (black boxes) along with renal failure evidenced by an increase in serum blood urea nitrogen (BUN) (F, *), which is prevented by orlistat depicted as †. Rat peripheral blood mononuclear cells from the control group (G), those treated with IL-1b+KC/GRO (200 ng/mL each for 30 min; H), 10 μM oleic acid (OA) for 10 min (I) or 30 min (J) were stained for annexin V and propidium iodide (PI) and analysed by flow cytometry. OA significantly increased (*) early apoptosis at 10 min (K), which progressed to predominantly necro-apoptosis by 30 min (L) with progressively increasing necrosis (M).