Figure 1. Brightness mode (B-mode) ultrasound images showed dark regions under the cortex 15 min post-IR.
Male BALB/c mice were subject to 28-min of bilateral ischemia by clamping the renal pedicles. Animals were imaged with B-mode ultrasound at 15-, 30-, 45-, 60 min and 24 hr after reperfusion. At 15 minutes post-IR a dark region was found under the cortex (C) in the examined animals. The dark band was not seen in any pre-IR or 24 hr post-IR image. Control animals not exposed to IR did not show the dark band at any time point.