The regional perfusion curves show that the microvascular perfusion decreased the most in the outer medulla during the first hour post-IR and its recovery took longer time compared to the cortex or to the inner medulla. Blue=cortex, Green=outer medulla, Red=inner medulla. Regions of interest selection on parametric maps and on B-mode images are shown in the lower panel of the figure. The region of interest selection was performed on the parametric perfusion maps (left panel) and confirmed on B-mode and contrast images at pre-IR, 15 min post-IR and 24 hr post-IR. The selected regions were maintained (placed automatically) on consecutive images (15-, 30-, 45-, and 60 min post-IR). The right panel shows an overlay of the region selectioned onto a photograph of the freshly excised parasagittal view of the mouse kidney.