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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Transplantation. 2016 Feb;100(2):325–333. doi: 10.1097/TP.0000000000000961

TABLE 1.

Nucleotide sequences of the different primers applied for the detection of renin, collagen I and GAPDH by real-time RT-PCR

Gene Primer pairs Product
length, bp
Renin forward: 5′-TGC CCA CCC TCC CCG ACA TT-3′ 167
reverse: 5′-GGC ACC CAG GAC CCA GAC AGG-3′
Collagen I forward: 5′-CTG CCC CGG CGC CGA AGT C-3′ 96
reverse: 5′-CCC TCG ACG CCG GTG GTT TCT TG-3′
GAPDH forward: 5′-ATC TGA CGT GCC GCC TGG AGA AAC-3′ 164
reverse: 5′-CCC GGC ATC GAA GGT GGA AGA GT-3′

GAPDH indicates glyceraldehyde-3-phosphate dehydrogenase.