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. Author manuscript; available in PMC: 2016 May 17.
Published in final edited form as: Tissue Eng Part C Methods. 2016 Jan 18;22(3):208–220. doi: 10.1089/ten.TEC.2015.0365

FIG. 5.

FIG. 5

Immuno-fluorescence analysis of collagen I and collagen II content within 2D- and 3D-expanded BMSC-seeded collagen scaffolds. BMSCs were isolated and expanded within 2D and 3D environments and differentiated within collagen scaffolds for 21 days in chondrogenic medium. Thereafter, constructs were fixed, sectioned at 5 μm thickness, and processed for visualization of cells (DAPI), collagen I (Col I; Texas Red) and collagen II (Col II; FITC). Presented photomicrographs represent cell–scaffold constructs derived from (A) 2D-expanded BMSCs seeded at 50, 10, 5, 1, or 0.5 × 106 BMSCs/cm3, and (B) 3D-expanded BMSCs seeded at 50, 10, 5, 1, or 0.5 × 106 BMNCs/cm3 (cells from donor Z28; 10× magnification). DAPI, 4′,6-diamidino-2-phenylindole. FITC, fluorescein isothiocyanate. Color images available online at www.liebertpub.com/tec