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. 2016 May 21;22(19):4662–4672. doi: 10.3748/wjg.v22.i19.4662

Table 1.

Details pertaining to primary and secondary antibodies used in immunofluorescence and Western blot

Host species Monoclonal/Polyclonal Antigen Product code Manufacturer Dilution
Rabbit Polyclonal Tryptophan hydroxylase 2 NB-100-74555 Novus biologicals (Cambridge, United Kingdom) 1:100
Mouse Monoclonal TMEM-16A (ANO1) Ab190721 Abcam (Cambridge, United Kingdom) 1:200
Mouse Monoclonal HuD sc-48421 Santa-Cruz Biotechnologies (Heidelberg, Germany) 1:100
Mouse Monoclonal Platelet-derived growth factor alpha sc-21789 Santa-Cruz Biotechnologies (Heidelberg, Germany) 1:100
Goat Polyclonal Neuronal nitric oxide synthase ab1376 Abcam (Cambridge, United Kingdom) 1:300
Mouse Monoclonal Vasoactive intestinal peptide sc-25347 Santa-Cruz Biotechnologies (Heidelberg, Germany) 1:100
Mouse Monoclonal Choline acetyltransferase ab49382 Abcam (Cambridge, United Kingdom) 1:100
Mouse Monoclonal GAPDH ab9484 Abcam (Cambridge, United Kingdom) 1:2000
Rabbit Polyclonal Mouse (secondary) ab6728 Abcam (Cambridge, United Kingdom) 1:10000
Donkey Polyclonal Rabbit (secondary) ab6802 Abcam (Cambridge, United Kingdom) 1:10000
Donkey Polyclonal Rabbit (Alexa Fluor® 488) ab150073 Abcam (Cambridge, United Kingdom) 1:500
Goat Polyclonal Mouse (Alexa Fluor® 594) ab150116 Abcam (Cambridge, United Kingdom) 1:500
Donkey Polyclonal Goat (Alexa Fluor® 555) ab150134 Abcam (Cambridge, United Kingdom) 1:500