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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: J Thromb Haemost. 2016 Mar 7;14(5):1070–1081. doi: 10.1111/jth.13245

Fig. 1.

Fig. 1

Platelet-targeting thiol isomerase sensitive fluorogenic sensor (PDI-sAb). Customized thiol containing peptides have either a quencher (CPQ2) attached at N-terminus and or a fluorophore (CP488) and an azido group attached at N-terminus and C-terminus, respectively. The fluorophore is quenched due to close proximity to quencher once the peptides were bridged correctly by disulfide bond. Dibenzylcyclooctyne-NHS ester (DBCO) labels primary amine groups on anti-CD61 antibody and reacts with azide-labeled peptide thus serving as a connector linking disulfide bridged peptides (PDI-sP) to antibody. Fluorescence increases (at 519 nm) upon disulfide bond breakage by thiol isomerase.