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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: J Thromb Haemost. 2016 Mar 7;14(5):1070–1081. doi: 10.1111/jth.13245

Fig. 2.

Fig. 2

PDI-sAb sensitivity to reducing reagents. To determine the sensitivity of synthesized PDI-sAb to reducing reagents, various concentrations of DTT (A) or GSH (B) were added to PDI-sAb (○ 5 μM, ■ 50 μM, □ 500 μM, ◆ 5 mM, ● 30 mM). Fluorescence increased immediately after the addition of reducing reagents and reached peak value within the first 30 seconds. Fold increase in RFU at 300 sec (C) reveals a difference in potency of the reducing reagents. (*, p < 0.05; **, p < 0.01, ***, p < 0.005)