Fig. 4.
Sensitivity of PDI-sAb to platelet-derived thiol reductase activity. Real-time flow cytometry of PDI-sAb labeled resting (A) or stimulated platelets (B). A rapid increase in PDI-sAb mean fluorescence was only observed when platelets were stimulated by TRAP (60 μM) and convulxin (2 nM). Data from all donors (mean ± SD) shows a significant increase in both thiol reductase activity and P-selectin expression on activated platelets after 10 min incubation (C). (**, p<0.01; ***, p<0.005)